Total Flavonoid Content and Antioxidant Activity by Different Drying and Extraction Methods of Clinacanthus nutans Leaves

• Main Authors: Mohd Hafiz Abd Majid, Yvonne Melse Laurence, Mohammad Shaheen Khan, Mohd Sani Sarjadi
• Format: Article
• Language: English
• Published: 2018
• Subjects: QK Botany; SB Plant culture
• Online Access: https://eprints.ums.edu.my/id/eprint/24092/1/Total%20Flavonoid%20Content%20and%20Antioxidant%20Activity%20by%20Different%20Drying%20and%20Extraction%20Methods%20of%20Clinacanthus%20nutans%20Leaves.pdf; https://eprints.ums.edu.my/id/eprint/24092/
• Institution: Universiti Malaysia Sabah

This report presents a study on the total flavonoid content and antioxidant activity of Clinacanthus nutans leaves from different drying and extraction methods. The C. nutans leaves were subjected through three different drying methods: oven (40°C, 60°C and 80°C), vacuum oven and air drying. Afterwards, extraction on the dried leaves was performed using three different extraction techniques: soxhlet, maceration and ultrasoundassisted solvent extraction. Finally, total flavonoid and antioxidantactivity was determined spectrophotometrically by aluminium chloride colorimetric assay and 2-Diphenyl-1-picryl hydrazyl (DPPH) method respectively. The results showed that the choice of drying and extraction methods had a significant influence on total flavonoid and antioxidant content of C. nutans extract. The combination of drying C. nutans leaves using laboratory oven at 60°C and soxhlet extraction obtained the highest amount of total flavonoid and antioxidant content at 24.53 ± 0.95 mg RU/g and 89.73 ± 4.39mg TE/ g respectively. The highest antioxidant activity for C. nutansfromair-dried samples and vacuum oven-dried samples were obtained through maceration extraction at 85.46 mg TE/ g and 83.96 mg TE/ g respectively.