Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats
Meat adulteration has been a signifcant issue in today’s food industry as it intertwine with religious, social and economic values. PCR based techniques for the detection of meat species in a meat admixture are primarily used by the industry as a reliable approach due to its sensitivity and reliabil...
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Universiti Putra Malaysia
2018
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my.unimas.ir.204312019-07-12T07:09:29Z http://ir.unimas.my/id/eprint/20431/ Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats Chung, H.H Q Science (General) QH Natural history QH301 Biology Meat adulteration has been a signifcant issue in today’s food industry as it intertwine with religious, social and economic values. PCR based techniques for the detection of meat species in a meat admixture are primarily used by the industry as a reliable approach due to its sensitivity and reliability. This paper describes the design and verifcation of real-time polymerase chain reaction (RT-PCR) based assay for the detection of meat from various non- target species by using species specifc oligonucleotides. Five sets of species-specifc primers have been developed to target small regions (≤ 150 bp) of the mitochondrial D-loop. The specifcity, sensitivity and reliability of each assay have been verifed by using SYBR Green based RT-PCR. By using a cut-off CT of 30 cycles, all assays show sensitivity down to 0.05% of the DNA spike level. When applied to DNA templates from raw meat admixtures, assays were able to detect the target species up to a level of 0.1%. Hence, this verify the potential applicability of these assays in the meat industry. Universiti Putra Malaysia 2018-04 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/20431/7/Real-time%20polymerase%20chain%20reaction%20%28abstract%29.pdf Chung, H.H (2018) Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats. International Food Research Journal, 25 (2). pp. 632-637. ISSN 2231 7546 http://www.ifrj.upm.edu.my/ifrj-2018-25-issue-2.html |
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Q Science (General) QH Natural history QH301 Biology Chung, H.H Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats |
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Meat adulteration has been a signifcant issue in today’s food industry as it intertwine with religious, social and economic values. PCR based techniques for the detection of meat species in a meat admixture are primarily used by the industry as a reliable approach due to its sensitivity and reliability. This paper describes the design and verifcation of real-time polymerase chain reaction (RT-PCR) based assay for the detection of meat from various non- target species by using species specifc oligonucleotides. Five sets of species-specifc primers have been developed to target small regions (≤ 150 bp) of the mitochondrial D-loop. The specifcity, sensitivity and reliability of each assay have been verifed by using SYBR Green based RT-PCR. By using a cut-off CT of 30 cycles, all assays show sensitivity down to 0.05% of the DNA spike level. When applied to DNA templates from raw meat admixtures, assays were able to detect the target species up to a level of 0.1%. Hence, this verify the potential applicability of these assays in the meat industry. |
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E-Article |
author |
Chung, H.H |
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Chung, H.H |
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Chung, H.H |
title |
Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats |
title_short |
Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats |
title_full |
Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats |
title_fullStr |
Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats |
title_full_unstemmed |
Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats |
title_sort |
real-time polymerase chain reaction (rt-pcr) for the authentication of raw meats |
publisher |
Universiti Putra Malaysia |
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2018 |
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http://ir.unimas.my/id/eprint/20431/7/Real-time%20polymerase%20chain%20reaction%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/20431/ http://www.ifrj.upm.edu.my/ifrj-2018-25-issue-2.html |
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