Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism
The inactivation of antinutritional factors, protease inhibitors within winged bean protein was induced by two respective method treatments. The physical method based on steam vapor that was conducted using an autoclave and chemical method consisting on pH-gradients of buffer solutions prepared at r...
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2022
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my.upm.eprints.1021882023-07-11T04:27:27Z http://psasir.upm.edu.my/id/eprint/102188/ Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism Saadi, Sami Saari, Nazamid Mohd Ghazali, Hasanah Abdulkarim, Mohammed Sabo The inactivation of antinutritional factors, protease inhibitors within winged bean protein was induced by two respective method treatments. The physical method based on steam vapor that was conducted using an autoclave and chemical method consisting on pH-gradients of buffer solutions prepared at respective acidic pH, neutral pH and alkaline pH ranges. The activity of remaining protease inhibitors of bowman birk inhibitor (BBI), and kunitz-trypsin inhibitor (KTI) after and before treatments was enzymatically confirmed using relevant antagonistic trypsin and combined trypsin-α-chymotrypsin digests. The resulting molecular assembly indicating an interval molecular relaxation range of °0.16 < °DA < °0.2 corresponding to reconformation in protein units with volume-mass changes of −2.17 < ∂v' < +2.17 and with denaturation/unfolding efficiency based on heat capacity ΔCp of 36.36 < DE/UF% < 54.67. These structural changes had a great benefit in determining and producing functional protein hydrolysates. Elsevier BV 2022 Article PeerReviewed Saadi, Sami and Saari, Nazamid and Mohd Ghazali, Hasanah and Abdulkarim, Mohammed Sabo (2022) Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism. Current Research in Food Science, 5. 207 - 221. ISSN 2665-9271 https://www.sciencedirect.com/science/article/pii/S2665927122000119 10.1016/j.crfs.2022.01.011 |
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The inactivation of antinutritional factors, protease inhibitors within winged bean protein was induced by two respective method treatments. The physical method based on steam vapor that was conducted using an autoclave and chemical method consisting on pH-gradients of buffer solutions prepared at respective acidic pH, neutral pH and alkaline pH ranges. The activity of remaining protease inhibitors of bowman birk inhibitor (BBI), and kunitz-trypsin inhibitor (KTI) after and before treatments was enzymatically confirmed using relevant antagonistic trypsin and combined trypsin-α-chymotrypsin digests. The resulting molecular assembly indicating an interval molecular relaxation range of °0.16 < °DA < °0.2 corresponding to reconformation in protein units with volume-mass changes of −2.17 < ∂v' < +2.17 and with denaturation/unfolding efficiency based on heat capacity ΔCp of 36.36 < DE/UF% < 54.67. These structural changes had a great benefit in determining and producing functional protein hydrolysates. |
| format |
Article |
| author |
Saadi, Sami Saari, Nazamid Mohd Ghazali, Hasanah Abdulkarim, Mohammed Sabo |
| spellingShingle |
Saadi, Sami Saari, Nazamid Mohd Ghazali, Hasanah Abdulkarim, Mohammed Sabo Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism |
| author_facet |
Saadi, Sami Saari, Nazamid Mohd Ghazali, Hasanah Abdulkarim, Mohammed Sabo |
| author_sort |
Saadi, Sami |
| title |
Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism |
| title_short |
Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism |
| title_full |
Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism |
| title_fullStr |
Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism |
| title_full_unstemmed |
Mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism |
| title_sort |
mitigation of antinutritional factors and protease inhibitors of defatted winged bean-seed proteins using thermal and hydrothermal treatments: denaturation/unfolding coupled hydrolysis mechanism |
| publisher |
Elsevier BV |
| publishDate |
2022 |
| url |
http://psasir.upm.edu.my/id/eprint/102188/ https://www.sciencedirect.com/science/article/pii/S2665927122000119 |
| _version_ |
1772813429516533760 |