Quantitation of green fluorescent protein using a gel-based imaging method.

• Main Authors: Chew, Few Ne, Tan, Wen Siang, Ling, Tau Chuan, Tan, Chon Seng, Tey, Beng Ti
• Format: Article
• Language: English; English
• Published: Elsevier Masson 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/16409/1/Quantitation%20of%20green%20fluorescent%20protein%20using%20a%20gel.pdf; http://psasir.upm.edu.my/id/eprint/16409/
• Institution: Universiti Putra Malaysia

Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the qua...