Quantitation of green fluorescent protein using a gel-based imaging method.
Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the qua...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier Masson
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16409/1/Quantitation%20of%20green%20fluorescent%20protein%20using%20a%20gel.pdf http://psasir.upm.edu.my/id/eprint/16409/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English English |
| Summary: | Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the quantitation of GFP was developed. The assay was evaluated for its precision, linearity, reproducibility, and sensitivity in the presence of Escherichia coli cells and was compared with the spectrofluorometric method. Using this newly established, gel-based imaging technique; the amount of GFP can be quantified accurately.
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