Quantitation of green fluorescent protein using a gel-based imaging method.

• Main Authors: Chew, Few Ne, Tan, Wen Siang, Ling, Tau Chuan, Tan, Chon Seng, Tey, Beng Ti
• Format: Article
• Language: English; English
• Published: Elsevier Masson 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/16409/1/Quantitation%20of%20green%20fluorescent%20protein%20using%20a%20gel.pdf; http://psasir.upm.edu.my/id/eprint/16409/
• Institution: Universiti Putra Malaysia

Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the quantitation of GFP was developed. The assay was evaluated for its precision, linearity, reproducibility, and sensitivity in the presence of Escherichia coli cells and was compared with the spectrofluorometric method. Using this newly established, gel-based imaging technique; the amount of GFP can be quantified accurately.