Biochemical and molecular identification of Enterococcus spp. from red pitaya

Biochemical and molecular identification of Enterococcus spp. from red pitaya

Red pitaya is a popular fruit worldwide due to its unique appearance and high nutrient contents. Many studies about the nutritional content and the physico-chemical characteristics of red pitaya have been performed but microorganisms that occurred naturally in the fruit remained unknown. Therefore,...

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Main Authors: Ong, Yien Yien, Tan, Wen Siang, Mohamad, Rosfarizan, Sieo, Chin Chin, Tey, Beng Ti
Format: Article
Published: Elsevier 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36644/
http://www.sciencedirect.com/science/article/pii/S1359511314000609
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.366442015-09-04T12:35:14Z http://psasir.upm.edu.my/id/eprint/36644/ Biochemical and molecular identification of Enterococcus spp. from red pitaya Ong, Yien Yien Tan, Wen Siang Mohamad, Rosfarizan Sieo, Chin Chin Tey, Beng Ti Red pitaya is a popular fruit worldwide due to its unique appearance and high nutrient contents. Many studies about the nutritional content and the physico-chemical characteristics of red pitaya have been performed but microorganisms that occurred naturally in the fruit remained unknown. Therefore, this study was conducted to characterize the enterococci present in red pitaya. Enterococci were isolated from red pitaya fruit and characterized with biochemical tests, randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) of the 16S rDNA region. A total of 30 bacteria were isolated from red pitaya, of which 20 isolates were confirmed as enterococci, genus Enterococcus. 13 isolates were further confirmed as Enterococcus casseliflavus and 7 were identified as Enterococcus gallinarum. Elsevier 2014-04 Article PeerReviewed Ong, Yien Yien and Tan, Wen Siang and Mohamad, Rosfarizan and Sieo, Chin Chin and Tey, Beng Ti (2014) Biochemical and molecular identification of Enterococcus spp. from red pitaya. Process Biochemistry, 49 (4). pp. 563-568. ISSN 1359-5113; ESSN: 1873-3298 http://www.sciencedirect.com/science/article/pii/S1359511314000609 10.1016/j.procbio.2014.01.019
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Red pitaya is a popular fruit worldwide due to its unique appearance and high nutrient contents. Many studies about the nutritional content and the physico-chemical characteristics of red pitaya have been performed but microorganisms that occurred naturally in the fruit remained unknown. Therefore, this study was conducted to characterize the enterococci present in red pitaya. Enterococci were isolated from red pitaya fruit and characterized with biochemical tests, randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) of the 16S rDNA region. A total of 30 bacteria were isolated from red pitaya, of which 20 isolates were confirmed as enterococci, genus Enterococcus. 13 isolates were further confirmed as Enterococcus casseliflavus and 7 were identified as Enterococcus gallinarum.
format Article
author Ong, Yien Yien
Tan, Wen Siang
Mohamad, Rosfarizan
Sieo, Chin Chin
Tey, Beng Ti
spellingShingle Ong, Yien Yien
Tan, Wen Siang
Mohamad, Rosfarizan
Sieo, Chin Chin
Tey, Beng Ti
Biochemical and molecular identification of Enterococcus spp. from red pitaya
author_facet Ong, Yien Yien
Tan, Wen Siang
Mohamad, Rosfarizan
Sieo, Chin Chin
Tey, Beng Ti
author_sort Ong, Yien Yien
title Biochemical and molecular identification of Enterococcus spp. from red pitaya
title_short Biochemical and molecular identification of Enterococcus spp. from red pitaya
title_full Biochemical and molecular identification of Enterococcus spp. from red pitaya
title_fullStr Biochemical and molecular identification of Enterococcus spp. from red pitaya
title_full_unstemmed Biochemical and molecular identification of Enterococcus spp. from red pitaya
title_sort biochemical and molecular identification of enterococcus spp. from red pitaya
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36644/
http://www.sciencedirect.com/science/article/pii/S1359511314000609
_version_ 1643831795029901312

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