Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia

Aim: The aim of this study was to determine the prevalence of various Salmonella serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant. Materials and Methods: A total of 182 poultry and environmental samples wer...

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Main Authors: Hafiz, Nidaullah, Nadarajan, Abirami, Ahamed Kamal, Shamila Syuhada, Chuah, Li Oon, Huda, Nurul, Tan, Teik Pei, Zainal Abidin, Farah Wahida, Rusul, Gulam
Format: Article
Language:English
Published: Veterinary World 2017
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Online Access:http://eprints.usm.my/37162/1/%28Prevalence_of_Salmonella_in_poultry%29__4.pdf
http://eprints.usm.my/37162/
https://www.ncbi.nlm.nih.gov/pubmed/28435190
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Institution: Universiti Sains Malaysia
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spelling my.usm.eprints.37162 http://eprints.usm.my/37162/ Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia Hafiz, Nidaullah Nadarajan, Abirami Ahamed Kamal, Shamila Syuhada Chuah, Li Oon Huda, Nurul Tan, Teik Pei Zainal Abidin, Farah Wahida Rusul, Gulam T1-995 Technology(General) Aim: The aim of this study was to determine the prevalence of various Salmonella serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant. Materials and Methods: A total of 182 poultry and environmental samples were collected at random on separate occasions from wet markets and small scale processing plant, during the period of October 2014 to July 2015 in Penang and Perlis, Malaysia. The samples were analyzed for the presence of Salmonella using ISO 6579:2002 conventional culture-based method. Presumptive Salmonella colonies were subjected to various biochemical tests (such as triple sugar iron and lysine iron test), serologically confirmed using polyvalent O and H antisera and further serotyped at Public Health Laboratory, Ministry of Health, Perak, Malaysia. Results: Salmonella serotypes were isolated from 161 out of 182 samples (88.46%) with 100% prevalence in the whole chicken carcass and chicken cuts - as well as transport crate, cage, drum, knife, chopping board, display table, floor, bench wash water, wash water, and drain water. Salmonella was isolated from 91.67%, 83.33%, and 66.67% of defeathering machines, drain swabs, and apron, respectively. 17 serotypes were isolated in this study with Salmonella Albany (57/161), Salmonella Corvallis (42/161), and Salmonella Brancaster (37/161) being the predominant serovars. Conclusion: The most carcass contact and environmental samples collected along the wet market chicken processing line were consistently contaminated with Salmonella. This indicates that Salmonella has established itself in poultry processing environments by colonizing the surfaces of the equipment and survives in these environments by establishing biofilms. Our results highlight the need of implementing strict hygiene and sanitation standards to reduce the incidence of Salmonella. The prevalence of Salmonella in poultry can be reduced effectively by identifying and eliminating the sources and contamination sites during slaughter and processing of poultry. Veterinary World 2017-03 Article PeerReviewed application/pdf en http://eprints.usm.my/37162/1/%28Prevalence_of_Salmonella_in_poultry%29__4.pdf Hafiz, Nidaullah and Nadarajan, Abirami and Ahamed Kamal, Shamila Syuhada and Chuah, Li Oon and Huda, Nurul and Tan, Teik Pei and Zainal Abidin, Farah Wahida and Rusul, Gulam (2017) Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia. Veterinary World, 10 (3). pp. 286-292. ISSN 0972-8988 https://www.ncbi.nlm.nih.gov/pubmed/28435190
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic T1-995 Technology(General)
spellingShingle T1-995 Technology(General)
Hafiz, Nidaullah
Nadarajan, Abirami
Ahamed Kamal, Shamila Syuhada
Chuah, Li Oon
Huda, Nurul
Tan, Teik Pei
Zainal Abidin, Farah Wahida
Rusul, Gulam
Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
description Aim: The aim of this study was to determine the prevalence of various Salmonella serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant. Materials and Methods: A total of 182 poultry and environmental samples were collected at random on separate occasions from wet markets and small scale processing plant, during the period of October 2014 to July 2015 in Penang and Perlis, Malaysia. The samples were analyzed for the presence of Salmonella using ISO 6579:2002 conventional culture-based method. Presumptive Salmonella colonies were subjected to various biochemical tests (such as triple sugar iron and lysine iron test), serologically confirmed using polyvalent O and H antisera and further serotyped at Public Health Laboratory, Ministry of Health, Perak, Malaysia. Results: Salmonella serotypes were isolated from 161 out of 182 samples (88.46%) with 100% prevalence in the whole chicken carcass and chicken cuts - as well as transport crate, cage, drum, knife, chopping board, display table, floor, bench wash water, wash water, and drain water. Salmonella was isolated from 91.67%, 83.33%, and 66.67% of defeathering machines, drain swabs, and apron, respectively. 17 serotypes were isolated in this study with Salmonella Albany (57/161), Salmonella Corvallis (42/161), and Salmonella Brancaster (37/161) being the predominant serovars. Conclusion: The most carcass contact and environmental samples collected along the wet market chicken processing line were consistently contaminated with Salmonella. This indicates that Salmonella has established itself in poultry processing environments by colonizing the surfaces of the equipment and survives in these environments by establishing biofilms. Our results highlight the need of implementing strict hygiene and sanitation standards to reduce the incidence of Salmonella. The prevalence of Salmonella in poultry can be reduced effectively by identifying and eliminating the sources and contamination sites during slaughter and processing of poultry.
format Article
author Hafiz, Nidaullah
Nadarajan, Abirami
Ahamed Kamal, Shamila Syuhada
Chuah, Li Oon
Huda, Nurul
Tan, Teik Pei
Zainal Abidin, Farah Wahida
Rusul, Gulam
author_facet Hafiz, Nidaullah
Nadarajan, Abirami
Ahamed Kamal, Shamila Syuhada
Chuah, Li Oon
Huda, Nurul
Tan, Teik Pei
Zainal Abidin, Farah Wahida
Rusul, Gulam
author_sort Hafiz, Nidaullah
title Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_short Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_full Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_fullStr Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_full_unstemmed Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_sort prevalence of salmonella in poultry processing environments in wet markets in penang and perlis, malaysia
publisher Veterinary World
publishDate 2017
url http://eprints.usm.my/37162/1/%28Prevalence_of_Salmonella_in_poultry%29__4.pdf
http://eprints.usm.my/37162/
https://www.ncbi.nlm.nih.gov/pubmed/28435190
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