Comparison of the protein content of Phaseolus vulgaris L. (abitsuwelas) using three methods of storage

• Main Authors: Guevara, Karlaarni M., Salinas, Louie Fe F.
• Format: text
• Language: English
• Published: Animo Repository 1992
• Subjects: Common bean > Storage; Food—Protein content; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/3926
• Institution: De La Salle University

The protein content of the pods and seeds of Phaseolus vulgaris L. (abitsuwelas) in the three methods used, namely: storage in a refrigerator, in a bamboo crate (kaing), and air-drying in a flat bamboo basket (bilao) for a period of seven days, were analyzed using the Bradford protein dye-binding method.Standard calibration curves were obtained by using Bovine Serum Albumin (BSA). Using the spectrophotometer at 595 nanometers, absorbance readings were recorded. Data were analyzed using the one-way analysis of variance for a completely randomized design. The difference between the treatment means were determined using the least significant difference (LSD) test.The results showed that the decrease in protein concentration of abitsuwelas pods stored in the refrigerator was not significantly different from the control until the third day, but became highly significant on the fourth day of storage. In contrast, the protein content of the pods stored in the bamboo crate and flat bamboo basket was not significant until the second day of storage, but became highly significant on the third day. As for the seeds, the protein content of those stored in the refrigerator was not significantly different from the control until the third day, but became significantly different on the fourth day, and highly significant on the fifth day of storage. In the case of the seeds stored in the flat-bamboo basket and bamboo crate, the protein content was not significantly different on the third day but became highly significantly different from the control on the fourth day "of storage."