Synthesis and structure-activity relationship of a microcionamide-inspired peptide

Here we describe the facile synthesis of four microcionamide-inspired peptides where the atypical 2- phenylethylenamine in the marine natural product, microcionamide A, was substituted by a similarly-aromatic and more easily incorporated tryptophan residue. Compounds 1-4 were synthesized using a sta...

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Main Authors: Inocentes, Carl Rogel V., Salvador-Reyes, Lilibeth A., Villaraza, Aaron Joseph L.
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Published: Animo Repository 2019
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/11430
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Institution: De La Salle University
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-116542024-01-15T05:47:10Z Synthesis and structure-activity relationship of a microcionamide-inspired peptide Inocentes, Carl Rogel V. Salvador-Reyes, Lilibeth A. Villaraza, Aaron Joseph L. Here we describe the facile synthesis of four microcionamide-inspired peptides where the atypical 2- phenylethylenamine in the marine natural product, microcionamide A, was substituted by a similarly-aromatic and more easily incorporated tryptophan residue. Compounds 1-4 were synthesized using a standard Fmoc-based solid-phase synthesis strategy followed by iodine-mediated on-resin cyclization for disulfide-bridged compounds 1-3. Biological activity evaluation revealed the strong antibacterial activity of compound 1 with MIC of 9.1 µM and 15 µM against S. aureus and P. aeruginosa, respectively. The inactivity of alanine analogues 2-4 against these pathogens suggests that the N-terminal Val, the cyclic scaffold, the contiguous lie residues and consequently, the hydrophobicity of compound 1 are essential for its antibacterial action. Compound 1 also favorably showed limited cytotoxicity against normal mammalian cell lines. Collectively, we have synthesized an analogue of microcionamide A, where replacement of the 2- phenylethylenamine moiety with a Trp residue retained the antibacterial activity and favorably diminished cytotoxicity. 2019-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/11430 Faculty Research Work Animo Repository Peptides Chemistry
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Peptides
Chemistry
spellingShingle Peptides
Chemistry
Inocentes, Carl Rogel V.
Salvador-Reyes, Lilibeth A.
Villaraza, Aaron Joseph L.
Synthesis and structure-activity relationship of a microcionamide-inspired peptide
description Here we describe the facile synthesis of four microcionamide-inspired peptides where the atypical 2- phenylethylenamine in the marine natural product, microcionamide A, was substituted by a similarly-aromatic and more easily incorporated tryptophan residue. Compounds 1-4 were synthesized using a standard Fmoc-based solid-phase synthesis strategy followed by iodine-mediated on-resin cyclization for disulfide-bridged compounds 1-3. Biological activity evaluation revealed the strong antibacterial activity of compound 1 with MIC of 9.1 µM and 15 µM against S. aureus and P. aeruginosa, respectively. The inactivity of alanine analogues 2-4 against these pathogens suggests that the N-terminal Val, the cyclic scaffold, the contiguous lie residues and consequently, the hydrophobicity of compound 1 are essential for its antibacterial action. Compound 1 also favorably showed limited cytotoxicity against normal mammalian cell lines. Collectively, we have synthesized an analogue of microcionamide A, where replacement of the 2- phenylethylenamine moiety with a Trp residue retained the antibacterial activity and favorably diminished cytotoxicity.
format text
author Inocentes, Carl Rogel V.
Salvador-Reyes, Lilibeth A.
Villaraza, Aaron Joseph L.
author_facet Inocentes, Carl Rogel V.
Salvador-Reyes, Lilibeth A.
Villaraza, Aaron Joseph L.
author_sort Inocentes, Carl Rogel V.
title Synthesis and structure-activity relationship of a microcionamide-inspired peptide
title_short Synthesis and structure-activity relationship of a microcionamide-inspired peptide
title_full Synthesis and structure-activity relationship of a microcionamide-inspired peptide
title_fullStr Synthesis and structure-activity relationship of a microcionamide-inspired peptide
title_full_unstemmed Synthesis and structure-activity relationship of a microcionamide-inspired peptide
title_sort synthesis and structure-activity relationship of a microcionamide-inspired peptide
publisher Animo Repository
publishDate 2019
url https://animorepository.dlsu.edu.ph/faculty_research/11430
_version_ 1789485840253059072