Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines

MicroRNAs (miRNAs) have recently been shown to play fundamental roles in diverse cellular processes and linked to variety of cancers. Dicer and Drosha are two major enzymes in the miRNA maturation process. DGCR8 is the assistant of Drosha in the microprocessor complex. In this study, we evaluated th...

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Main Authors: Jafari, Naser, Peeri Dogaheh, Hadi, Bohlooli, Shahab, Oyong, Glenn G., Shirzad, Zohreh, Alibeiki, Fatemeh, Hosseini Asl, Saeid, Jalal Zargar, Seyed
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Published: Animo Repository 2013
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/2450
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Institution: De La Salle University
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-34492021-09-01T01:27:25Z Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines Jafari, Naser Peeri Dogaheh, Hadi Bohlooli, Shahab Oyong, Glenn G. Shirzad, Zohreh Alibeiki, Fatemeh Hosseini Asl, Saeid Jalal Zargar, Seyed MicroRNAs (miRNAs) have recently been shown to play fundamental roles in diverse cellular processes and linked to variety of cancers. Dicer and Drosha are two major enzymes in the miRNA maturation process. DGCR8 is the assistant of Drosha in the microprocessor complex. In this study, we evaluated the mRNA expression profiles of major miRNA processing machinery Drosha, Dicer, and DGCR8 in human gastrointestinal (AGS, KYSE30 and HepG2) cancer cell lines. Materials and Methods: The cells were cultured and harvested, and total cellular RNA was isolated from cells. Then, first-strand cDNA was synthesized from the RNA of cells. Afterward, Quantitative analysis was performed by real-time RT-PCR using the PowerSYBR Green PCR Master Mix. Results: Expression levels of Drosha in AGS and HepG2 cells were higher than the controls, whereas, Drosha's expression level in KYSE-30 cell line was lower. The Dicer expression levels in AGS and HepG2 cells were higher, while, its expression level in KYSE-30 cell was lower. The DGCR8 expression levels in all three cell lines were significantly higher than the control samples. Conclusion: Expression levels of the two most important enzymes of the miRNA machinery, Drosha and Dicer, and microprocessor complex component, DGCR8 were noticeably dysregulated when compared to healthy controls. 2013-04-30T07:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/2450 Faculty Research Work Animo Repository MicroRNA Cancer cells—Growth RNA-protein interactions Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic MicroRNA
Cancer cells—Growth
RNA-protein interactions
Biology
spellingShingle MicroRNA
Cancer cells—Growth
RNA-protein interactions
Biology
Jafari, Naser
Peeri Dogaheh, Hadi
Bohlooli, Shahab
Oyong, Glenn G.
Shirzad, Zohreh
Alibeiki, Fatemeh
Hosseini Asl, Saeid
Jalal Zargar, Seyed
Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines
description MicroRNAs (miRNAs) have recently been shown to play fundamental roles in diverse cellular processes and linked to variety of cancers. Dicer and Drosha are two major enzymes in the miRNA maturation process. DGCR8 is the assistant of Drosha in the microprocessor complex. In this study, we evaluated the mRNA expression profiles of major miRNA processing machinery Drosha, Dicer, and DGCR8 in human gastrointestinal (AGS, KYSE30 and HepG2) cancer cell lines. Materials and Methods: The cells were cultured and harvested, and total cellular RNA was isolated from cells. Then, first-strand cDNA was synthesized from the RNA of cells. Afterward, Quantitative analysis was performed by real-time RT-PCR using the PowerSYBR Green PCR Master Mix. Results: Expression levels of Drosha in AGS and HepG2 cells were higher than the controls, whereas, Drosha's expression level in KYSE-30 cell line was lower. The Dicer expression levels in AGS and HepG2 cells were higher, while, its expression level in KYSE-30 cell was lower. The DGCR8 expression levels in all three cell lines were significantly higher than the control samples. Conclusion: Expression levels of the two most important enzymes of the miRNA machinery, Drosha and Dicer, and microprocessor complex component, DGCR8 were noticeably dysregulated when compared to healthy controls.
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author Jafari, Naser
Peeri Dogaheh, Hadi
Bohlooli, Shahab
Oyong, Glenn G.
Shirzad, Zohreh
Alibeiki, Fatemeh
Hosseini Asl, Saeid
Jalal Zargar, Seyed
author_facet Jafari, Naser
Peeri Dogaheh, Hadi
Bohlooli, Shahab
Oyong, Glenn G.
Shirzad, Zohreh
Alibeiki, Fatemeh
Hosseini Asl, Saeid
Jalal Zargar, Seyed
author_sort Jafari, Naser
title Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines
title_short Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines
title_full Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines
title_fullStr Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines
title_full_unstemmed Expression levels of microRNA machinery components Drosha, dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines
title_sort expression levels of microrna machinery components drosha, dicer and dgcr8 in human (ags, hepg2, and keyse-30) cancer cell lines
publisher Animo Repository
publishDate 2013
url https://animorepository.dlsu.edu.ph/faculty_research/2450
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