Electrophoretic profiles of water and salt-soluble proteins from the seeds of citrus species as genetic markers

• Main Authors: Janairo, Jose Isagani B., Saulog, K. F., Llanos, Nancy Lazaro
• Format: text
• Published: Animo Repository 2008
• Subjects: Genetic markers; Citrus fruits—Seeds; Biochemistry, Biophysics, and Structural Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/6601
• Institution: De La Salle University

The use of seed proteins in the evaluation of genetic diversity is cost effective and less time consuming. Water and salt-soluble proteins from the seeds of citrus species: C reticulate, C. aurantifolia, C grandis, C. sinensis, C. limon, and C. microcarpa were extracted and variations in electrophoretic profiles were determined. Finely grounded seeds were homogenized in 50 mM Tris buffer, pH = 7.0, centrifuged at 14,000 rpm for 10 minutes at 40 C, and the supernate collected as the water-soluble fraction. The pellet was resuspended in 50mM Tris buffer, pH = 7.0 in 150 mM NaCl, homogenized, then centrifuged at 14,000 rpm for 10 minutes at 40 C, and the supernate collected as teh salt-soluble fraction. Samples were analyzed using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. Nine protein bands were found common to all samples and six bands were used as genetic markers in the water-soluble fractions. In the salt-soluble fraction, ten protein band were found to be common to all samples and five bands were concluded to be genetic markers. C. reticulata and C. sinensis possessed identical water and salt soluble protein profiles and C. grandis was found to be most distinct among the samples.