Preparation and Binding Evaluation of Histamine-Imprinted Microspheres via Conventional Thermal and RAFT-Mediated Free-Radical Polymerization

• Main Authors: Romano, Edwin F, Jr., So, Regina C, Donne, Scott W, Holdsworth, Clovia I
• Format: text
• Published: Archīum Ateneo 2016
• Subjects: Polymer particles; Carbon fiber; Genetics; Polymers; Microspheres; Chemistry; Food Science; Polymer Chemistry
• Online Access: https://archium.ateneo.edu/chemistry-faculty-pubs/175; https://pubs.acs.org/doi/10.1021/acsomega.6b00144
• Institution: Ateneo De Manila University

Elevated histamine (HTM) levels are closely linked to food poisoning as well as to pathophysiological allergic diseases. In this study, HTM-imprinted, solution-processable microspheres were prepared via high-dilution conventional thermal polymerization (CTP) and controlled radical polymerization (CRP) using ethylene glycol dimethacrylate (80 or 90 wt %) and methacrylic acid at 60 °C in acetonitrile and evaluated as recognition materials for sensing applications. The polymers were selective to HTM in binding studies, cross-rebinding, and competitive binding assays against the HTM analogues histidine, imidazole, and tryptamine. The selective binding capacity was significantly higher with CTP-80 (on the basis of mass: 21.0 μmol/g and surface area: 8.08 × 10–2 μmol/m2) than that with both CTP-90 (8.47 μmol/g, 4.49 × 10–2 μmol/m2) and CRP-80 (9.00 μmol/g, 1.19 × 10–2 μmol/m2).