Widespread recognition of 5' splice sites by noncanonical base-pairing to U1 snRNA involving bulged nucleotides

• Main Authors: Bennett, C. Frank, Roca, Xavier, Akerman, Martin, Gaus, Hans, Berdeja, Andrés, Krainer, Adrian R.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/100017; http://hdl.handle.net/10220/16284
• Institution: Nanyang Technological University

An established paradigm in pre-mRNA splicing is the recognition of the 5′ splice site (5′ss) by canonical base-pairing to the 5′ end of U1 small nuclear RNA (snRNA). We recently reported that a small subset of 5′ss base-pair to U1 in an alternate register that is shifted by 1 nucleotide. Using genet...