Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while me...
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sg-ntu-dr.10356-1000312022-02-16T16:28:44Z Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George James Lee Kong Chian School of Medicine (LKCMedicine) Research Techno Plaza DRNTU::Science::Medicine We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions. Published version 2014-06-11T08:19:59Z 2019-12-06T20:15:28Z 2014-06-11T08:19:59Z 2019-12-06T20:15:28Z 2014 2014 Journal Article Kim, J., Lee, S., Tsuda, S., Zhang, X., Asrican, B., Gloss, B., et al. (2014). Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses. Cell Reports, 7, 1-13. 2211-1247 https://hdl.handle.net/10356/100031 http://hdl.handle.net/10220/19679 10.1016/j.celrep.2014.04.047 24857665 en Cell reports This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). © 2014 The Authors. Published by Elsevier Inc. User rights governed by an Open Access license. application/pdf |
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DRNTU::Science::Medicine Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George James Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
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We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions. |
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Lee Kong Chian School of Medicine (LKCMedicine) |
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Lee Kong Chian School of Medicine (LKCMedicine) Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George James |
format |
Article |
author |
Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George James |
author_sort |
Kim, Jinsook |
title |
Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
title_short |
Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
title_full |
Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
title_fullStr |
Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
title_full_unstemmed |
Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
title_sort |
optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses |
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2014 |
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https://hdl.handle.net/10356/100031 http://hdl.handle.net/10220/19679 |
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1725985582767669248 |