Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses

We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while me...

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Main Authors: Kim, Jinsook, Lee, Soojung, Tsuda, Sachiko, Zhang, Xuying, Asrican, Brent, Gloss, Bernd, Feng, Guoping, Augustine, George James
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/100031
http://hdl.handle.net/10220/19679
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1000312022-02-16T16:28:44Z Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses Kim, Jinsook Lee, Soojung Tsuda, Sachiko Zhang, Xuying Asrican, Brent Gloss, Bernd Feng, Guoping Augustine, George James Lee Kong Chian School of Medicine (LKCMedicine) Research Techno Plaza DRNTU::Science::Medicine We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions. Published version 2014-06-11T08:19:59Z 2019-12-06T20:15:28Z 2014-06-11T08:19:59Z 2019-12-06T20:15:28Z 2014 2014 Journal Article Kim, J., Lee, S., Tsuda, S., Zhang, X., Asrican, B., Gloss, B., et al. (2014). Optogenetic Mapping of Cerebellar Inhibitory Circuitry Reveals Spatially Biased Coordination of Interneurons via Electrical Synapses. Cell Reports, 7, 1-13. 2211-1247 https://hdl.handle.net/10356/100031 http://hdl.handle.net/10220/19679 10.1016/j.celrep.2014.04.047 24857665 en Cell reports This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). © 2014 The Authors. Published by Elsevier Inc. User rights governed by an Open Access license. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Medicine
spellingShingle DRNTU::Science::Medicine
Kim, Jinsook
Lee, Soojung
Tsuda, Sachiko
Zhang, Xuying
Asrican, Brent
Gloss, Bernd
Feng, Guoping
Augustine, George James
Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
description We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Kim, Jinsook
Lee, Soojung
Tsuda, Sachiko
Zhang, Xuying
Asrican, Brent
Gloss, Bernd
Feng, Guoping
Augustine, George James
format Article
author Kim, Jinsook
Lee, Soojung
Tsuda, Sachiko
Zhang, Xuying
Asrican, Brent
Gloss, Bernd
Feng, Guoping
Augustine, George James
author_sort Kim, Jinsook
title Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
title_short Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
title_full Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
title_fullStr Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
title_full_unstemmed Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
title_sort optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses
publishDate 2014
url https://hdl.handle.net/10356/100031
http://hdl.handle.net/10220/19679
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