Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the th...

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Bibliographic Details
Main Authors: Yu, Yong, Liu, Jianzhao, Zhao, Zujin, Ng, Ka Ming, Luo, Kathy Qian, Tang, Ben Zhong
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/101256
http://hdl.handle.net/10220/11069
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Institution: Nanyang Technological University
Language: English
Description
Summary:A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.

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