Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the th...

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Main Authors: Yu, Yong, Liu, Jianzhao, Zhao, Zujin, Ng, Ka Ming, Luo, Kathy Qian, Tang, Ben Zhong
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/101256
http://hdl.handle.net/10220/11069
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1012562020-03-07T11:35:31Z Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit Yu, Yong Liu, Jianzhao Zhao, Zujin Ng, Ka Ming Luo, Kathy Qian Tang, Ben Zhong School of Chemical and Biomedical Engineering A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit. 2013-07-09T07:08:47Z 2019-12-06T20:35:43Z 2013-07-09T07:08:47Z 2019-12-06T20:35:43Z 2012 2012 Journal Article Yu, Y., Liu, J., Zhao, Z., Ng, K. M., Luo, K. Q., Tang, B. Z. (2012). Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit. Chemical Communications, 48(51), 6360-6362. https://hdl.handle.net/10356/101256 http://hdl.handle.net/10220/11069 10.1039/c2cc32038j en Chemical communications © 2012 The Royal Society of Chemistry.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Yu, Yong
Liu, Jianzhao
Zhao, Zujin
Ng, Ka Ming
Luo, Kathy Qian
Tang, Ben Zhong
format Article
author Yu, Yong
Liu, Jianzhao
Zhao, Zujin
Ng, Ka Ming
Luo, Kathy Qian
Tang, Ben Zhong
spellingShingle Yu, Yong
Liu, Jianzhao
Zhao, Zujin
Ng, Ka Ming
Luo, Kathy Qian
Tang, Ben Zhong
Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
author_sort Yu, Yong
title Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
title_short Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
title_full Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
title_fullStr Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
title_full_unstemmed Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
title_sort facile preparation of non-self-quenching fluorescent dna strands with the degree of labeling up to the theoretic limit
publishDate 2013
url https://hdl.handle.net/10356/101256
http://hdl.handle.net/10220/11069
_version_ 1681040446383456256

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