Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

• Main Authors: Yu, Yong, Liu, Jianzhao, Zhao, Zujin, Ng, Ka Ming, Luo, Kathy Qian, Tang, Ben Zhong
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2013
• Online Access: https://hdl.handle.net/10356/101256; http://hdl.handle.net/10220/11069
• Institution: Nanyang Technological University

A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the th...