Pick one, but be quick : 5' splice sites and the problems of too many choices

Splice site selection is fundamental to pre-mRNA splicing and the expansion of genomic coding potential. 5′ Splice sites (5′ss) are the critical elements at the 5′ end of introns and are extremely diverse, as thousands of different sequences act as bona fide 5′ss in the human transcriptome. Most 5′s...

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Main Authors: Roca, Xavier, Krainer, Adrian R., Eperon, Ian C.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/101529
http://hdl.handle.net/10220/18670
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1015292023-02-28T16:56:47Z Pick one, but be quick : 5' splice sites and the problems of too many choices Roca, Xavier Krainer, Adrian R. Eperon, Ian C. School of Biological Sciences DRNTU::Science::Biological sciences Splice site selection is fundamental to pre-mRNA splicing and the expansion of genomic coding potential. 5′ Splice sites (5′ss) are the critical elements at the 5′ end of introns and are extremely diverse, as thousands of different sequences act as bona fide 5′ss in the human transcriptome. Most 5′ss are recognized by base-pairing with the 5′ end of the U1 small nuclear RNA (snRNA). Here we review the history of research on 5′ss selection, highlighting the difficulties of establishing how base-pairing strength determines splicing outcomes. We also discuss recent work demonstrating that U1 snRNA:5′ss helices can accommodate noncanonical registers such as bulged duplexes. In addition, we describe the mechanisms by which other snRNAs, regulatory proteins, splicing enhancers, and the relative positions of alternative 5′ss contribute to selection. Moreover, we discuss mechanisms by which the recognition of numerous candidate 5′ss might lead to selection of a single 5′ss and propose that protein complexes propagate along the exon, thereby changing its physical behavior so as to affect 5′ss selection. Published version 2014-01-22T02:34:54Z 2019-12-06T20:40:01Z 2014-01-22T02:34:54Z 2019-12-06T20:40:01Z 2013 2013 Journal Article Roca, X., Krainer, A. R., & Eperon, I. C. (2013). Pick one, but be quick : 5' splice sites and the problems of too many choices. Genes & development, 27(2), 129-144. 0890-9369 https://hdl.handle.net/10356/101529 http://hdl.handle.net/10220/18670 10.1101/gad.209759.112 23348838 en Genes & development © 2013 The Author(s). This paper was published in Genes & Development and is made available as an electronic reprint (preprint) with permission of The Author(s). The paper can be found at the following official DOI: [http://dx.doi.org/10.1101/gad.209759.112]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Roca, Xavier
Krainer, Adrian R.
Eperon, Ian C.
Pick one, but be quick : 5' splice sites and the problems of too many choices
description Splice site selection is fundamental to pre-mRNA splicing and the expansion of genomic coding potential. 5′ Splice sites (5′ss) are the critical elements at the 5′ end of introns and are extremely diverse, as thousands of different sequences act as bona fide 5′ss in the human transcriptome. Most 5′ss are recognized by base-pairing with the 5′ end of the U1 small nuclear RNA (snRNA). Here we review the history of research on 5′ss selection, highlighting the difficulties of establishing how base-pairing strength determines splicing outcomes. We also discuss recent work demonstrating that U1 snRNA:5′ss helices can accommodate noncanonical registers such as bulged duplexes. In addition, we describe the mechanisms by which other snRNAs, regulatory proteins, splicing enhancers, and the relative positions of alternative 5′ss contribute to selection. Moreover, we discuss mechanisms by which the recognition of numerous candidate 5′ss might lead to selection of a single 5′ss and propose that protein complexes propagate along the exon, thereby changing its physical behavior so as to affect 5′ss selection.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Roca, Xavier
Krainer, Adrian R.
Eperon, Ian C.
format Article
author Roca, Xavier
Krainer, Adrian R.
Eperon, Ian C.
author_sort Roca, Xavier
title Pick one, but be quick : 5' splice sites and the problems of too many choices
title_short Pick one, but be quick : 5' splice sites and the problems of too many choices
title_full Pick one, but be quick : 5' splice sites and the problems of too many choices
title_fullStr Pick one, but be quick : 5' splice sites and the problems of too many choices
title_full_unstemmed Pick one, but be quick : 5' splice sites and the problems of too many choices
title_sort pick one, but be quick : 5' splice sites and the problems of too many choices
publishDate 2014
url https://hdl.handle.net/10356/101529
http://hdl.handle.net/10220/18670
_version_ 1759857994114269184