Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures

Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures

Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid s...

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Bibliographic Details
Main Authors: Li, Huaqiong, Wong, Yee Shan, Wen, Feng, Ng, Kee Woei, Ng, Gary Ka Lai, Venkatraman, Subbu S., Boey, Freddy Yin Chiang, Tan, Lay Poh
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2014
Subjects:
DRNTU::Science::Biological sciences::Molecular biology
Online Access:https://hdl.handle.net/10356/101674
http://hdl.handle.net/10220/19695
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Institution: Nanyang Technological University
Language: English
Description
Summary:Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds.

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