Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation

• Main Authors: Grimley, Joshua S., Li, Li, Wang, Weina, Wen, Lei, Beese, Lorena S., Hellinga, Homme W., Augustine, George James
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Science::Medicine::Biomedical engineering
• Online Access: https://hdl.handle.net/10356/102024; http://hdl.handle.net/10220/18902
• Institution: Nanyang Technological University

We describe an engineered fluorescent optogenetic sensor, SuperClomeleon, that robustly detects inhibitory synaptic activity in single, cultured mouse neurons by reporting intracellular chloride changes produced by exogenous GABA or inhibitory synaptic activity. Using a cell-free protein engineering...