Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation

• Main Authors: Grimley, Joshua S., Li, Li, Wang, Weina, Wen, Lei, Beese, Lorena S., Hellinga, Homme W., Augustine, George James
• 其他作者: Lee Kong Chian School of Medicine (LKCMedicine)
• 格式: Article
• 語言: English
• 出版: 2014
• 主題: DRNTU::Science::Medicine::Biomedical engineering
• 在線閱讀: https://hdl.handle.net/10356/102024; http://hdl.handle.net/10220/18902
• 機構: Nanyang Technological University

We describe an engineered fluorescent optogenetic sensor, SuperClomeleon, that robustly detects inhibitory synaptic activity in single, cultured mouse neurons by reporting intracellular chloride changes produced by exogenous GABA or inhibitory synaptic activity. Using a cell-free protein engineering...