Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation

• المؤلفون الرئيسيون: Grimley, Joshua S., Li, Li, Wang, Weina, Wen, Lei, Beese, Lorena S., Hellinga, Homme W., Augustine, George James
• مؤلفون آخرون: Lee Kong Chian School of Medicine (LKCMedicine)
• التنسيق: مقال
• اللغة: English
• منشور في: 2014
• الموضوعات: DRNTU::Science::Medicine::Biomedical engineering
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/102024; http://hdl.handle.net/10220/18902

We describe an engineered fluorescent optogenetic sensor, SuperClomeleon, that robustly detects inhibitory synaptic activity in single, cultured mouse neurons by reporting intracellular chloride changes produced by exogenous GABA or inhibitory synaptic activity. Using a cell-free protein engineering...