A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression

• Main Authors: Makhija, Harshyaa, Roy, Suki, Hoon, Shawn, Ghadessy, Farid John, Wong, Desmond, Jaiswal, Rahul, Campana, Dario, Dröge, Peter
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: Transgenesis; Protein Expression; DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/103690; http://hdl.handle.net/10220/47376
• Institution: Nanyang Technological University

Advances in stem cell engineering, gene therapy and molecular medicine often involve genome engineering at a cellular level. However, functionally large or multi transgene cassette insertion into the human genome still remains a challenge. Current practices such as random transgene integration or ta...