Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications

• Main Authors: Heidari, Amir, Yoon, Yong-Jin, Park, Woo-Tae, Su, Pei-Chen, Miao, Jianmin, Park, Mi Kyoung, Lin, Julius Tsai Ming
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Mechanical engineering::Bio-mechatronics
• Online Access: https://hdl.handle.net/10356/104974; http://hdl.handle.net/10220/20369
• Institution: Nanyang Technological University

Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.