Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications
Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The reson...
Saved in:
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2014
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/104974 http://hdl.handle.net/10220/20369 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-104974 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-1049742023-03-04T17:20:39Z Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications Heidari, Amir Yoon, Yong-Jin Park, Woo-Tae Su, Pei-Chen Miao, Jianmin Park, Mi Kyoung Lin, Julius Tsai Ming School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering::Bio-mechatronics Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests. Published version 2014-08-21T06:31:55Z 2019-12-06T21:43:52Z 2014-08-21T06:31:55Z 2019-12-06T21:43:52Z 2014 2014 Journal Article Heidari, A., Yoon, Y.-J., Park, W.-T., Su, P.-C., Miao, J., Lin, J. T. M., et al. (2014). Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications. Sensors, 14(3), 4585-4598. 1424-8220 https://hdl.handle.net/10356/104974 http://hdl.handle.net/10220/20369 10.3390/s140304585 24608003 en Sensors © 2014 by the authors; licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
DRNTU::Engineering::Mechanical engineering::Bio-mechatronics |
spellingShingle |
DRNTU::Engineering::Mechanical engineering::Bio-mechatronics Heidari, Amir Yoon, Yong-Jin Park, Woo-Tae Su, Pei-Chen Miao, Jianmin Park, Mi Kyoung Lin, Julius Tsai Ming Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications |
description |
Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests. |
author2 |
School of Mechanical and Aerospace Engineering |
author_facet |
School of Mechanical and Aerospace Engineering Heidari, Amir Yoon, Yong-Jin Park, Woo-Tae Su, Pei-Chen Miao, Jianmin Park, Mi Kyoung Lin, Julius Tsai Ming |
format |
Article |
author |
Heidari, Amir Yoon, Yong-Jin Park, Woo-Tae Su, Pei-Chen Miao, Jianmin Park, Mi Kyoung Lin, Julius Tsai Ming |
author_sort |
Heidari, Amir |
title |
Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications |
title_short |
Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications |
title_full |
Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications |
title_fullStr |
Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications |
title_full_unstemmed |
Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications |
title_sort |
biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications |
publishDate |
2014 |
url |
https://hdl.handle.net/10356/104974 http://hdl.handle.net/10220/20369 |
_version_ |
1759858205578493952 |