Automated assessment of β-cell area and density per islet and patient using TMEM27 and BACE2 immunofluorescence staining in human pancreatic β-cells

• Main Authors: Rechsteiner, Markus P., Floros, Xenofon, Boehm, Bernhard O., Marselli, Lorella, Marchetti, Piero, Stoffel, Markus, Moch, Holger, Spinas, Giatgen A.
• Other Authors: Pietropaolo, Massimo
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Science::Medicine
• Online Access: https://hdl.handle.net/10356/105082; http://hdl.handle.net/10220/20420
• Institution: Nanyang Technological University

In this study we aimed to establish an unbiased automatic quantification pipeline to assess islet specific features such as β-cell area and density per islet based on immunofluorescence stainings. To determine these parameters, the in vivo protein expression levels of TMEM27 and BACE2 in pancreatic...