Effect of time discretization of the imaging process on the accuracy of trajectory estimation in fluorescence microscopy

• Main Authors: Lin, Zhiping, Ober, Raimund J., Wong, Yau, Chao, Jerry
• Other Authors: School of Electrical and Electronic Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Materials
• Online Access: https://hdl.handle.net/10356/105352; http://hdl.handle.net/10220/20673
• Institution: Nanyang Technological University

In fluorescence microscopy, high-speed imaging is often necessary for the proper visualization and analysis of fast subcellular dynamics. Here, we examine how the speed of image acquisition affects the accuracy with which parameters such as the starting position and speed of a microscopic non-statio...