Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells

• Main Authors: Zheng, Qiupeng, Cai, Xiaohong, Tan, Meng How, Schaffert, Steven, Arnold, Christopher P., Gong, Xue, Chen, Chang-Zheng, Huang, Shenglin
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2019
• Subjects: Genome Editing; DRNTU::Engineering::Bioengineering; CRISPR/Cas9
• Online Access: https://hdl.handle.net/10356/106786; http://hdl.handle.net/10220/48994
• Institution: Nanyang Technological University

The prokaryotic type II CRISPR/Cas9 system has been adapted to perform targeted genome editing in cells and model organisms. Here, we describe targeted gene deletion and replacement in human cells via the CRISPR/Cas9 system using two guide RNAs. The system effectively generated targeted deletions of...