Differential association of chromatin proteins identifies BAF60a/SMARCD1 as a regulator of embryonic stem cell differentiation

• Main Authors: Alajem, Adi, Biran, Alva, Harikumar, Arigela, Sailaja, Badi Sri, Aaronson, Yair, Livyatan, Ilana, Nissim-Rafinia, Malka, Sommer, Andreia Gianotti, Mostoslavsky, Gustavo, Gerbasi, Vincent R., Golden, Daniel E., Datta, Arnab, Sze, Siu Kwan, Meshorer, Eran
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/107289; http://hdl.handle.net/10220/25451
• Institution: Nanyang Technological University

Embryonic stem cells (ESCs) possess a distinct chromatin conformation maintained by specialized chromatin proteins. To identify chromatin regulators in ESCs, we developed a simple biochemical assay named D-CAP (differential chromatin-associated proteins), using brief micrococcal nuclease digestion o...