Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level

• Main Authors: Deng, Yibing, Wu, Tao, Wang, Mengdi, Shi, Shengchao, Yuan, Guodong, Li, Xi, Chong, Hanchung, Wu, Bin, Zheng, Peng
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: Atomic Force Microscopy; Peptides; Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/107449; http://hdl.handle.net/10220/49733
• Institution: Nanyang Technological University

The recent development of chemical and bio-conjugation techniques allows for the engineering of various protein polymers. However, most of the polymerization process is difficult to control. To meet this challenge, we develop an enzymatic procedure to build polyprotein using the combination of a str...