Parallel alignment of bacteria using near-field optical force array for cell sorting

This paper presents a near-field approach to align multiple rod-shaped bacteria based on the interference pattern in silicon nano-waveguide arrays. The bacteria in the optical field will be first trapped by the gradient force and then rotated by the scattering force to the equilibrium position. In t...

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Main Authors: Zhao, Hai Tao, Zhang, Yi, Chin, Lip Ket, Yap, Peng Huat, Wang, K., Ser, Wee, Liu, Ai Qun
Other Authors: School of Electrical and Electronic Engineering
Format: Conference or Workshop Item
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/138926
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1389262020-05-14T03:12:49Z Parallel alignment of bacteria using near-field optical force array for cell sorting Zhao, Hai Tao Zhang, Yi Chin, Lip Ket Yap, Peng Huat Wang, K. Ser, Wee Liu, Ai Qun School of Electrical and Electronic Engineering School of Mechanical and Aerospace Engineering Lee Kong Chian School of Medicine (LKCMedicine) Proceedings of SPIE - Optical Trapping and Optical Micromanipulation XIV 2017 Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics Rod-shaped Bacteria Near-field This paper presents a near-field approach to align multiple rod-shaped bacteria based on the interference pattern in silicon nano-waveguide arrays. The bacteria in the optical field will be first trapped by the gradient force and then rotated by the scattering force to the equilibrium position. In the experiment, the Shigella bacteria is rotated 90 deg and aligned to horizontal direction in 9.4 s. Meanwhile, ~150 Shigella is trapped on the surface in 5 min and 86% is aligned with angle < 5 deg. This method is a promising toolbox for the research of parallel single-cell biophysical characterization, cell-cell interaction, etc. NRF (Natl Research Foundation, S’pore) Published version 2020-05-14T03:12:49Z 2020-05-14T03:12:49Z 2017 Conference Paper Zhao, H. T., Zhang, Y., Chin, L. K., Yap, P. H., Wang, K., Ser, W., & Liu, A. Q. (2017). Parallel alignment of bacteria using near-field optical force array for cell sorting. Proceedings of SPIE - Optical Trapping and Optical Micromanipulation XIV 2017, 10347, 1034739-. doi:10.1117/12.2271939 9781510611511 https://hdl.handle.net/10356/138926 10.1117/12.2271939 2-s2.0-85034651890 10347 en Copyright 2017 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics
Rod-shaped Bacteria
Near-field
spellingShingle Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics
Rod-shaped Bacteria
Near-field
Zhao, Hai Tao
Zhang, Yi
Chin, Lip Ket
Yap, Peng Huat
Wang, K.
Ser, Wee
Liu, Ai Qun
Parallel alignment of bacteria using near-field optical force array for cell sorting
description This paper presents a near-field approach to align multiple rod-shaped bacteria based on the interference pattern in silicon nano-waveguide arrays. The bacteria in the optical field will be first trapped by the gradient force and then rotated by the scattering force to the equilibrium position. In the experiment, the Shigella bacteria is rotated 90 deg and aligned to horizontal direction in 9.4 s. Meanwhile, ~150 Shigella is trapped on the surface in 5 min and 86% is aligned with angle < 5 deg. This method is a promising toolbox for the research of parallel single-cell biophysical characterization, cell-cell interaction, etc.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Zhao, Hai Tao
Zhang, Yi
Chin, Lip Ket
Yap, Peng Huat
Wang, K.
Ser, Wee
Liu, Ai Qun
format Conference or Workshop Item
author Zhao, Hai Tao
Zhang, Yi
Chin, Lip Ket
Yap, Peng Huat
Wang, K.
Ser, Wee
Liu, Ai Qun
author_sort Zhao, Hai Tao
title Parallel alignment of bacteria using near-field optical force array for cell sorting
title_short Parallel alignment of bacteria using near-field optical force array for cell sorting
title_full Parallel alignment of bacteria using near-field optical force array for cell sorting
title_fullStr Parallel alignment of bacteria using near-field optical force array for cell sorting
title_full_unstemmed Parallel alignment of bacteria using near-field optical force array for cell sorting
title_sort parallel alignment of bacteria using near-field optical force array for cell sorting
publishDate 2020
url https://hdl.handle.net/10356/138926
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