A catalytic and selective scissoring molecular tool for quadruplex nucleic acids

A catalytic and selective scissoring molecular tool for quadruplex nucleic acids

A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry...

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Bibliographic Details
Main Authors: Nadai, Matteo, Doria, Filippo, Scalabrin, Matteo, Pirota, Valentina, Grande, Vincenzo, Bergamaschi, Greta, Amendola, Valeria, Winnerdy, Fernaldo Richtia, Phan, Anh Tuân, Richter, Sara N., Freccero, Mauro
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2020
Subjects:
Science::Biological sciences
Hydroxyls
Genetics
Online Access:https://hdl.handle.net/10356/139320
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Institution: Nanyang Technological University
Language: English
Description
Summary:A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes.

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