A catalytic and selective scissoring molecular tool for quadruplex nucleic acids

A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry...

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Main Authors:	Nadai, Matteo, Doria, Filippo, Scalabrin, Matteo, Pirota, Valentina, Grande, Vincenzo, Bergamaschi, Greta, Amendola, Valeria, Winnerdy, Fernaldo Richtia, Phan, Anh Tuân, Richter, Sara N., Freccero, Mauro
Other Authors:	School of Physical and Mathematical Sciences
Format:	Article
Language:	English
Published:	2020
Subjects:	Science::Biological sciences Hydroxyls Genetics
Online Access:	https://hdl.handle.net/10356/139320
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Institution:	Nanyang Technological University
Language:	English

id	sg-ntu-dr.10356-139320
record_format	dspace
spelling	sg-ntu-dr.10356-1393202023-02-28T20:01:08Z A catalytic and selective scissoring molecular tool for quadruplex nucleic acids Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro School of Physical and Mathematical Sciences Science::Biological sciences Hydroxyls Genetics A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes. Published version 2020-05-19T01:15:30Z 2020-05-19T01:15:30Z 2018 Journal Article Nadai, M., Doria, F., Scalabrin, M., Pirota, V., Grande, V., Bergamaschi, G., . . . Freccero, M. (2018). A catalytic and selective scissoring molecular tool for quadruplex nucleic acids. Journal of the American Chemical Society, 140(44), 14528-14532. doi:10.1021/jacs.8b05337 0002-7863 https://hdl.handle.net/10356/139320 10.1021/jacs.8b05337 30351011 2-s2.0-85055042631 44 140 14528 14532 en Journal of the American Chemical Society © 2018 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	Science::Biological sciences Hydroxyls Genetics
spellingShingle	Science::Biological sciences Hydroxyls Genetics Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro A catalytic and selective scissoring molecular tool for quadruplex nucleic acids
description	A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes.
author2	School of Physical and Mathematical Sciences
author_facet	School of Physical and Mathematical Sciences Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro
format	Article
author	Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro
author_sort	Nadai, Matteo
title	A catalytic and selective scissoring molecular tool for quadruplex nucleic acids
title_short	A catalytic and selective scissoring molecular tool for quadruplex nucleic acids
title_full	A catalytic and selective scissoring molecular tool for quadruplex nucleic acids
title_fullStr	A catalytic and selective scissoring molecular tool for quadruplex nucleic acids
title_full_unstemmed	A catalytic and selective scissoring molecular tool for quadruplex nucleic acids
title_sort	catalytic and selective scissoring molecular tool for quadruplex nucleic acids
publishDate	2020
url	https://hdl.handle.net/10356/139320
_version_	1759856062637277184

A catalytic and selective scissoring molecular tool for quadruplex nucleic acids

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