An unprecedented knot‐like G‐quadruplex peripheral motif

A knot‐like G‐quadruplex peripheral structure is formed by a 7‐nt DNA sequence DL7 (TGTTGGT), in which six out of its seven nucleobases participate in compact base‐pairing interactions. Here, the solution NMR structure of a 24‐nt DNA oligonucleotide containing the DL7 sequence shows the interaction...

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Main Authors: Truong, Thi Hong Anh, Winnerdy, Fernaldo Richtia, Phan, Anh Tuân
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2020
Subjects:
DNA
Online Access:https://hdl.handle.net/10356/140168
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1401682023-02-28T19:46:23Z An unprecedented knot‐like G‐quadruplex peripheral motif Truong, Thi Hong Anh Winnerdy, Fernaldo Richtia Phan, Anh Tuân School of Physical and Mathematical Sciences NTU Institute of Structural Biology Science::Chemistry Science::Biological sciences Base Pairing DNA A knot‐like G‐quadruplex peripheral structure is formed by a 7‐nt DNA sequence DL7 (TGTTGGT), in which six out of its seven nucleobases participate in compact base‐pairing interactions. Here, the solution NMR structure of a 24‐nt DNA oligonucleotide containing the DL7 sequence shows the interaction between a two‐layer anti‐parallel G‐quadruplex core and the peripheral knot‐like structure, including the construction of two sharp turns in the DNA backbone. The formation of this novel structural element highlights the intricate properties of single‐stranded DNA folding in presence of G‐quadruplex‐forming motifs. We demonstrated the compatibility of the DL7 knot‐like structure with various G‐quadruplexes, which could have implications in drug design and DNA engineering. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Accepted version 2020-05-27T03:38:01Z 2020-05-27T03:38:01Z 2019 Journal Article Truong, T. H. A., Winnerdy, F. R., & Phan, A. T. (2019). An unprecedented knot‐like G‐quadruplex peripheral motif. Angewandte Chemie International Edition, 58(39), 13834-13839. doi:10.1002/anie.201907740 1433-7851 https://hdl.handle.net/10356/140168 10.1002/anie.201907740 31317601 2-s2.0-85070916928 39 58 13834 13839 en NRF-NRFI2017-09 MOE2015-T2-1-092 Angewandte Chemie International Edition This is the accepted version of the following article: Truong, T. H. A., Winnerdy, F. R., & Phan, A. T. (2019). An unprecedented knot‐like G‐quadruplex peripheral motif. Angewandte Chemie International Edition, 58(39), 13834-13839. doi:10.1002/anie.201907740, which has been published in final form at https://doi.org/10.1002/anie.201907740. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Chemistry
Science::Biological sciences
Base Pairing
DNA
spellingShingle Science::Chemistry
Science::Biological sciences
Base Pairing
DNA
Truong, Thi Hong Anh
Winnerdy, Fernaldo Richtia
Phan, Anh Tuân
An unprecedented knot‐like G‐quadruplex peripheral motif
description A knot‐like G‐quadruplex peripheral structure is formed by a 7‐nt DNA sequence DL7 (TGTTGGT), in which six out of its seven nucleobases participate in compact base‐pairing interactions. Here, the solution NMR structure of a 24‐nt DNA oligonucleotide containing the DL7 sequence shows the interaction between a two‐layer anti‐parallel G‐quadruplex core and the peripheral knot‐like structure, including the construction of two sharp turns in the DNA backbone. The formation of this novel structural element highlights the intricate properties of single‐stranded DNA folding in presence of G‐quadruplex‐forming motifs. We demonstrated the compatibility of the DL7 knot‐like structure with various G‐quadruplexes, which could have implications in drug design and DNA engineering.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Truong, Thi Hong Anh
Winnerdy, Fernaldo Richtia
Phan, Anh Tuân
format Article
author Truong, Thi Hong Anh
Winnerdy, Fernaldo Richtia
Phan, Anh Tuân
author_sort Truong, Thi Hong Anh
title An unprecedented knot‐like G‐quadruplex peripheral motif
title_short An unprecedented knot‐like G‐quadruplex peripheral motif
title_full An unprecedented knot‐like G‐quadruplex peripheral motif
title_fullStr An unprecedented knot‐like G‐quadruplex peripheral motif
title_full_unstemmed An unprecedented knot‐like G‐quadruplex peripheral motif
title_sort unprecedented knot‐like g‐quadruplex peripheral motif
publishDate 2020
url https://hdl.handle.net/10356/140168
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