Functional elucidation of a novel protein tetratricopeptide.

• Main Author: Lin, Valerie.
• Other Authors: School of Biological Sciences
• Format: Research Report
• Language: English
• Published: 2008
• Subjects: DRNTU::Science::Biological sciences::Human anatomy and physiology::Deoxyribonucleic acids
• Online Access: http://hdl.handle.net/10356/14223
• Institution: Nanyang Technological University

TTC9 was first reported as a hypothetical protein KIAA0227 by Nagase et al based on the sequence analysis of a cDNA clone isolated from a brain cDNA library. It was also reported later by the National Institutes of MGC Program. It is now referred to as TTC9 because of its sequence homology to a family of TTC proteins which contain tetratricopeptide repeat (TPR) domains. Human TTC9 is located on chromosome 14q24.2. Its transcript sizes are predicted to be 5217 bp and 2567 bp respectively, both of which encode a predicted protein of 336 amino acids (aa). The updated NCBI entry (accession XM_027236.5) predicted a 5453 bp transcript that encodes a 415 aa protein. Using Vector NTI software: we deduced the ORF of TTC9 mRNA (XM_027236.5) to be from nucleotide 580 to 1248, encoding a protein of 222 aa that contains 3 TPR domains at its carboxyl terminus. Blast searches: using the amino acid sequence 1 - 51, which is outside of the TPR domains of TTC9, yielded no sequence similarity to any protein domains in the database, suggesting that TTC9 is a unique novel protein.