Tracking genome-editing and associated molecular perturbations by SWATH mass spectrometry

• Main Authors: Lin, Qifeng, Low, Larry W. L., Lau, Adam, Chua, Esther W. L., Matsuoka, Yuji, Lian, Yilong, Monteiro, Antónia, Tate, Stephen, Gunaratne, Jayantha, Carney, Tom J.
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Medicine; SWATH; Mass Spectrometry (MS)
• Online Access: https://hdl.handle.net/10356/142406
• Institution: Nanyang Technological University

Advances in gene editing now allow reverse genetics to be applied to a broad range of biological systems. Ultimately, any modification to coding sequences requires confirmation at the protein level, although immunoblotting is often hampered by antibody quality or availability especially in non-model...