The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells

The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells

Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block-poly 2-(dimethylamino)ethyl metha...

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Bibliographic Details
Main Authors: Cao, Zhonglin, Xiao, Huiyu, Li, Li, Liu, Maixian, Lin, Guimiao, Zhai, Peng, Yong, Ken-Tye, Wang, Xiaomei, Xu, Gaixia
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2020
Subjects:
Engineering::Electrical and electronic engineering
siRNA
QDs
Online Access:https://hdl.handle.net/10356/142452
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Institution: Nanyang Technological University
Language: English
Description
Summary:Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block-poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy.

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