The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells

Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block-poly 2-(dimethylamino)ethyl metha...

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Main Authors: Cao, Zhonglin, Xiao, Huiyu, Li, Li, Liu, Maixian, Lin, Guimiao, Zhai, Peng, Yong, Ken-Tye, Wang, Xiaomei, Xu, Gaixia
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2020
Subjects:
QDs
Online Access:https://hdl.handle.net/10356/142452
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1424522020-06-22T06:40:53Z The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells Cao, Zhonglin Xiao, Huiyu Li, Li Liu, Maixian Lin, Guimiao Zhai, Peng Yong, Ken-Tye Wang, Xiaomei Xu, Gaixia School of Electrical and Electronic Engineering Engineering::Electrical and electronic engineering siRNA QDs Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block-poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy. Published version 2020-06-22T06:40:53Z 2020-06-22T06:40:53Z 2019 Journal Article Cao, Z., Xiao, H., Li, L., Liu, M., Lin, G., Zhai, P., . . . Xu, G. (2019). The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells. Frontiers in Pharmacology, 10, 1194-. doi:10.3389/fphar.2019.01194 1663-9812 https://hdl.handle.net/10356/142452 10.3389/fphar.2019.01194 31680969 2-s2.0-85074214793 10 en Frontiers in Pharmacology © 2019 Cao, Xiao, Li, Liu, Lin, Zhai, Yong, Wang and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Engineering::Electrical and electronic engineering
siRNA
QDs
spellingShingle Engineering::Electrical and electronic engineering
siRNA
QDs
Cao, Zhonglin
Xiao, Huiyu
Li, Li
Liu, Maixian
Lin, Guimiao
Zhai, Peng
Yong, Ken-Tye
Wang, Xiaomei
Xu, Gaixia
The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells
description Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block-poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Cao, Zhonglin
Xiao, Huiyu
Li, Li
Liu, Maixian
Lin, Guimiao
Zhai, Peng
Yong, Ken-Tye
Wang, Xiaomei
Xu, Gaixia
format Article
author Cao, Zhonglin
Xiao, Huiyu
Li, Li
Liu, Maixian
Lin, Guimiao
Zhai, Peng
Yong, Ken-Tye
Wang, Xiaomei
Xu, Gaixia
author_sort Cao, Zhonglin
title The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells
title_short The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells
title_full The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells
title_fullStr The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells
title_full_unstemmed The codelivery of siRNA and QDs by pH-responsive micelle for hepatoma cancer cells
title_sort codelivery of sirna and qds by ph-responsive micelle for hepatoma cancer cells
publishDate 2020
url https://hdl.handle.net/10356/142452
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