λ-integrase mediated seamless vector transgenesis platform

• المؤلف الرئيسي: Roy, Suki
• مؤلفون آخرون: Peter Droge
• التنسيق: Thesis-Doctor of Philosophy
• اللغة: English
• منشور في: Nanyang Technological University 2020
• الموضوعات: Science::Biological sciences::Molecular biology
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/142767

Genome engineering is an important component of gene/cell therapy and molecular medicine. In this respect, several DNA editing tools, such as ZFNs, TALEN and CRISPR-Cas system are being currently used for transgenesis. However, issues like insertional mutagenesis, off-target events, and small payloa...