Localized delivery of CRISPR/dCas9 via layer-by-layer self-assembling peptide coating on nanofibers for neural tissue engineering

• Main Authors: Zhang, Kunyu, Chooi, Wai Hon, Liu, Shuang, Chin, Jiah Shin, Murray, Aoife, Nizetic, Dean, Cheng, Du, Chew, Sing Yian
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2020
• Subjects: Engineering::Chemical engineering; Gene Delivery; CRISPR/Cas9
• Online Access: https://hdl.handle.net/10356/143512
• Institution: Nanyang Technological University

The clustered regularly interspaced short palindromic repeat (CRISPR) systems have a wide variety of applications besides precise genome editing. In particular, the CRISPR/dCas9 system can be used to control specific gene expression by CRISPR activation (CRISPRa) or interference (CRISPRi). However,...