One-pot dual labeling of IgG 1 and preparation of C-to-C fusion proteins through a combination of sortase A and butelase 1

• Main Authors: Harmand, Thibault J., Bousbaine, Djenet, Chan, Alix, Zhang, Xiaohong, Liu, David R., Tam, James P., Ploegh, Hidde L.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Biological sciences::Biochemistry; Immunology; Peptides and Proteins
• Online Access: https://hdl.handle.net/10356/143935
• Institution: Nanyang Technological University

Site-specific chemical modification of proteins can assist in the study of their function. Furthermore, these methods are essential to develop biologicals for diagnostic and therapeutic use. Standard protein engineering protocols and recombinant expression enable the production of proteins with shor...