Outer-Membrane Protease (OmpT) based E. coli sensing with anionic polythiophene and unlabeled peptide substrate

• Main Authors: Sinsinbar, Gaurav, Gudlur, Sushanth, Wood, Sarah E., Ammanath, Gopal, Yildiz, Hakan U., Alagappan, Palaniappan, Mrksich, Milan, Liedberg, Bo
• Other Authors: School of Materials Science and Engineering
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Chemistry::Analytical chemistry::Proteins; Science::Biological sciences::Microbiology::Bacteria; Enzymes; Fluorescence
• Online Access: https://hdl.handle.net/10356/144268
• Institution: Nanyang Technological University

E. coli and Salmonella are two of the most common bacterial pathogens involved in food- and water-borne-related deaths. Hence, it is critical to develop rapid and sensitive detection strategies for near-outbreak applications. Reported is a simple and specific assay to detect as low as 1 CFUmL1 of E....