Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus

Nisin is applied as a food preservative in processed foods and has the potential to be used synergistically with antibiotics for treatment of patients infected by antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus. The present study explores the antimicrobial effect o...

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Main Authors: Jensen, Camilla, Li, Heng, Vestergaard, Martin, Dalsgaard, Anders, Frees, Dorte, Leisner, Jørgen J
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2020
Subjects:
TEM
Online Access:https://hdl.handle.net/10356/145227
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1452272023-12-29T06:49:47Z Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus Jensen, Camilla Li, Heng Vestergaard, Martin Dalsgaard, Anders Frees, Dorte Leisner, Jørgen J School of Chemical and Biomedical Engineering Science::Medicine Food Preservative TEM Nisin is applied as a food preservative in processed foods and has the potential to be used synergistically with antibiotics for treatment of patients infected by antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus. The present study explores the antimicrobial effect of nisin on S. aureus viability and membrane integrity and, for the first time, used super-resolution microscopy to study morphological changes induced in S. aureus cells exposed to nisin. The exposure of S. aureus to nisin caused membrane depolarization and rapid killing. Super-resolution structured-illumination microscopy and transmission electron microscopy confirmed that nisin damages the cellular membrane and causes lysis of cells. Strikingly, condensation of chromosomal DNA was observed in all cells exposed to nisin, a phenotype not previously reported for this compound. Moreover, cells exposed to nisin were significantly smaller than non-exposed cells indicating the emergence of cell shrinkage. The strong association of DNA condensation with nisin exposure indicates that nisin interferes with chromosome replication or segregation in S. aureus. Published version 2020-12-15T06:34:39Z 2020-12-15T06:34:39Z 2020 Journal Article Jensen, C., Li, H., Vestergaard, M., Dalsgaard, A., Frees, D., & Leisner, J. J. (2020). Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus. Frontiers in Microbiology. 11, 1007-. doi:10.3389/fmicb.2020.01007 1664-302X https://hdl.handle.net/10356/145227 10.3389/fmicb.2020.01007 32582052 11 en Frontiers in Microbiology © 2020 Jensen, Li, Vestergaard, Dalsgaard, Frees and Leisner. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Food Preservative
TEM
spellingShingle Science::Medicine
Food Preservative
TEM
Jensen, Camilla
Li, Heng
Vestergaard, Martin
Dalsgaard, Anders
Frees, Dorte
Leisner, Jørgen J
Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus
description Nisin is applied as a food preservative in processed foods and has the potential to be used synergistically with antibiotics for treatment of patients infected by antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus. The present study explores the antimicrobial effect of nisin on S. aureus viability and membrane integrity and, for the first time, used super-resolution microscopy to study morphological changes induced in S. aureus cells exposed to nisin. The exposure of S. aureus to nisin caused membrane depolarization and rapid killing. Super-resolution structured-illumination microscopy and transmission electron microscopy confirmed that nisin damages the cellular membrane and causes lysis of cells. Strikingly, condensation of chromosomal DNA was observed in all cells exposed to nisin, a phenotype not previously reported for this compound. Moreover, cells exposed to nisin were significantly smaller than non-exposed cells indicating the emergence of cell shrinkage. The strong association of DNA condensation with nisin exposure indicates that nisin interferes with chromosome replication or segregation in S. aureus.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Jensen, Camilla
Li, Heng
Vestergaard, Martin
Dalsgaard, Anders
Frees, Dorte
Leisner, Jørgen J
format Article
author Jensen, Camilla
Li, Heng
Vestergaard, Martin
Dalsgaard, Anders
Frees, Dorte
Leisner, Jørgen J
author_sort Jensen, Camilla
title Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus
title_short Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus
title_full Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus
title_fullStr Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus
title_full_unstemmed Nisin damages the septal membrane and triggers DNA condensation in methicillin-resistant Staphylococcus aureus
title_sort nisin damages the septal membrane and triggers dna condensation in methicillin-resistant staphylococcus aureus
publishDate 2020
url https://hdl.handle.net/10356/145227
_version_ 1787136643061252096