A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples

A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples

Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference...

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Bibliographic Details
Main Authors: Kucharski, Michal, Tripathi, Jaishree, Nayak, Sourav, Zhu, Lei, Wirjanata, Grennady, van der Pluijm, Rob W., Dhorda, Mehul, Dondorp, Arjen, Bozdech, Zbynek
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2021
Subjects:
Science::Medicine
Whole Transcriptome Analysis
RNA Extraction
Online Access:https://hdl.handle.net/10356/147976
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Institution: Nanyang Technological University
Language: English
Description
Summary:Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference protocol for the efficient generation of transcriptome data from growing number of samples. Here, a comprehensive methodology on how to preserve, extract, amplify, and sequence full-length mRNA transcripts from Plasmodium-infected blood samples is presented that can be fully streamlined for high-throughput studies.

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