A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples

Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference...

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Main Authors:	Kucharski, Michal, Tripathi, Jaishree, Nayak, Sourav, Zhu, Lei, Wirjanata, Grennady, van der Pluijm, Rob W., Dhorda, Mehul, Dondorp, Arjen, Bozdech, Zbynek
Other Authors:	School of Biological Sciences
Format:	Article
Language:	English
Published:	2021
Subjects:	Science::Medicine Whole Transcriptome Analysis RNA Extraction
Online Access:	https://hdl.handle.net/10356/147976
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Institution:	Nanyang Technological University
Language:	English

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spelling	sg-ntu-dr.10356-1479762023-02-28T17:09:03Z A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples Kucharski, Michal Tripathi, Jaishree Nayak, Sourav Zhu, Lei Wirjanata, Grennady van der Pluijm, Rob W. Dhorda, Mehul Dondorp, Arjen Bozdech, Zbynek School of Biological Sciences Science::Medicine Whole Transcriptome Analysis RNA Extraction Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference protocol for the efficient generation of transcriptome data from growing number of samples. Here, a comprehensive methodology on how to preserve, extract, amplify, and sequence full-length mRNA transcripts from Plasmodium-infected blood samples is presented that can be fully streamlined for high-throughput studies. Ministry of Education (MOE) National Medical Research Council (NMRC) Published version This study was funded by Singapore National Medical Research Council grant number NMRC/OFIRG/0040/2017 and AcRF Tier 2 grant from the Singapore Ministry of Education (#MOE2017-T2-2-030 (S)). 2021-04-29T01:13:44Z 2021-04-29T01:13:44Z 2020 Journal Article Kucharski, M., Tripathi, J., Nayak, S., Zhu, L., Wirjanata, G., van der Pluijm, R. W., Dhorda, M., Dondorp, A. & Bozdech, Z. (2020). A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples. Malaria Journal, 19(1). https://dx.doi.org/10.1186/s12936-020-03436-w 1475-2875 0000-0002-1505-5608 0000-0003-3461-4767 0000-0002-9830-8446 https://hdl.handle.net/10356/147976 10.1186/s12936-020-03436-w 33036628 2-s2.0-85092454499 1 19 en NMRC/OFIRG/0040/2017 MOE2017-T2-2-030 (S) Malaria Journal © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	Science::Medicine Whole Transcriptome Analysis RNA Extraction
spellingShingle	Science::Medicine Whole Transcriptome Analysis RNA Extraction Kucharski, Michal Tripathi, Jaishree Nayak, Sourav Zhu, Lei Wirjanata, Grennady van der Pluijm, Rob W. Dhorda, Mehul Dondorp, Arjen Bozdech, Zbynek A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples
description	Sequencing technology advancements opened new opportunities to use transcriptomics for studying malaria pathology and epidemiology. Even though in recent years the study of whole parasite transcriptome proved to be essential in understanding parasite biology there is no compiled up-to-date reference protocol for the efficient generation of transcriptome data from growing number of samples. Here, a comprehensive methodology on how to preserve, extract, amplify, and sequence full-length mRNA transcripts from Plasmodium-infected blood samples is presented that can be fully streamlined for high-throughput studies.
author2	School of Biological Sciences
author_facet	School of Biological Sciences Kucharski, Michal Tripathi, Jaishree Nayak, Sourav Zhu, Lei Wirjanata, Grennady van der Pluijm, Rob W. Dhorda, Mehul Dondorp, Arjen Bozdech, Zbynek
format	Article
author	Kucharski, Michal Tripathi, Jaishree Nayak, Sourav Zhu, Lei Wirjanata, Grennady van der Pluijm, Rob W. Dhorda, Mehul Dondorp, Arjen Bozdech, Zbynek
author_sort	Kucharski, Michal
title	A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples
title_short	A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples
title_full	A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples
title_fullStr	A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples
title_full_unstemmed	A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples
title_sort	comprehensive rna handling and transcriptomics guide for high-throughput processing of plasmodium blood-stage samples
publishDate	2021
url	https://hdl.handle.net/10356/147976
_version_	1759854132425916416

A comprehensive RNA handling and transcriptomics guide for high-throughput processing of Plasmodium blood-stage samples

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