Regulation of cell migration by BMP signaling

A morphogen is a soluble signaling molecule which instructs cellular processes in a concentration-dependent manner. Bone morphogenetic proteins (BMP) are morphogens essential for development and repair in both embryos and adults. Preliminary observations in our group suggested that BMP signaling may...

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Main Author: Leong, Leslie Boon Hao
Other Authors: Tom James Carney
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2021
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Online Access:https://hdl.handle.net/10356/149380
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1493802023-02-28T18:08:15Z Regulation of cell migration by BMP signaling Leong, Leslie Boon Hao Tom James Carney School of Biological Sciences Experimental Medicine Building, Lee Kong Chian School of Medicine tcarney@ntu.edu.sg Science::Biological sciences::Genetics A morphogen is a soluble signaling molecule which instructs cellular processes in a concentration-dependent manner. Bone morphogenetic proteins (BMP) are morphogens essential for development and repair in both embryos and adults. Preliminary observations in our group suggested that BMP signaling may play a role during fibroblast migration in the developing fin of zebrafish embryos. By introducing BMP inhibitors to chemically block BMP signaling, retardation of fibroblast migration in the fin was observed. However, the exact mechanism of BMP signaling in directing cell migration in the developing fin is still unclear. Therefore, the aim of this project is to further investigate the role of BMP signaling in directing cell migration in the fin. In addition to chemical inhibition, BMP signaling was disrupted genetically by establishing transgenic zebrafish that conditionally overexpresses dominant-negative form of BMPR1ba (DNBMPR1ba). This was followed by observing its effect on fibroblast migration in the fin. Our results indicate that disruption of BMP signaling genetically also result in retardation of fibroblast migration in the developing fin, thus implying the importance of BMP signaling in directing migration of mesodermal-derived fibroblasts in the fin. Bachelor of Science in Biological Sciences 2021-06-08T13:48:50Z 2021-06-08T13:48:50Z 2021 Final Year Project (FYP) Leong, L. B. H. (2021). Regulation of cell migration by BMP signaling. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/149380 https://hdl.handle.net/10356/149380 en SBS20-179 application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences::Genetics
spellingShingle Science::Biological sciences::Genetics
Leong, Leslie Boon Hao
Regulation of cell migration by BMP signaling
description A morphogen is a soluble signaling molecule which instructs cellular processes in a concentration-dependent manner. Bone morphogenetic proteins (BMP) are morphogens essential for development and repair in both embryos and adults. Preliminary observations in our group suggested that BMP signaling may play a role during fibroblast migration in the developing fin of zebrafish embryos. By introducing BMP inhibitors to chemically block BMP signaling, retardation of fibroblast migration in the fin was observed. However, the exact mechanism of BMP signaling in directing cell migration in the developing fin is still unclear. Therefore, the aim of this project is to further investigate the role of BMP signaling in directing cell migration in the fin. In addition to chemical inhibition, BMP signaling was disrupted genetically by establishing transgenic zebrafish that conditionally overexpresses dominant-negative form of BMPR1ba (DNBMPR1ba). This was followed by observing its effect on fibroblast migration in the fin. Our results indicate that disruption of BMP signaling genetically also result in retardation of fibroblast migration in the developing fin, thus implying the importance of BMP signaling in directing migration of mesodermal-derived fibroblasts in the fin.
author2 Tom James Carney
author_facet Tom James Carney
Leong, Leslie Boon Hao
format Final Year Project
author Leong, Leslie Boon Hao
author_sort Leong, Leslie Boon Hao
title Regulation of cell migration by BMP signaling
title_short Regulation of cell migration by BMP signaling
title_full Regulation of cell migration by BMP signaling
title_fullStr Regulation of cell migration by BMP signaling
title_full_unstemmed Regulation of cell migration by BMP signaling
title_sort regulation of cell migration by bmp signaling
publisher Nanyang Technological University
publishDate 2021
url https://hdl.handle.net/10356/149380
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