Construction of a G-quadruplex-specific DNA endonuclease

Construction of a G-quadruplex-specific DNA endonuclease

We generated a novel G-quadruplex (G4)-specific endonuclease by fusing a G4 recognition domain of the RHAU helicase with a cleavage domain of the Fok1 nuclease. The fusion protein can specifically bind a parallel G4 and cleave a double-stranded DNA (dsDNA) next to it. The new endonuclease could be u...

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Bibliographic Details
Main Authors: Dang, Dung Thanh, Nguyen, Le Tuan Anh, Truong, Tuom Tinh Thi, Nguyen, Hoang Duc, Phan, Anh Tuân
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2021
Subjects:
Science::Biological sciences::Molecular biology
Science::Chemistry::Biochemistry
G-quadruplex (G4)
dsDNA
Online Access:https://hdl.handle.net/10356/149750
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Institution: Nanyang Technological University
Language: English
Description
Summary:We generated a novel G-quadruplex (G4)-specific endonuclease by fusing a G4 recognition domain of the RHAU helicase with a cleavage domain of the Fok1 nuclease. The fusion protein can specifically bind a parallel G4 and cleave a double-stranded DNA (dsDNA) next to it. The new endonuclease could be used to detect a G4 in a long dsDNA, providing a useful tool for mapping the formation of G4s in the genome.

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