Construction of a G-quadruplex-specific DNA endonuclease

• Main Authors: Dang, Dung Thanh, Nguyen, Le Tuan Anh, Truong, Tuom Tinh Thi, Nguyen, Hoang Duc, Phan, Anh Tuân
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences::Molecular biology; Science::Chemistry::Biochemistry; G-quadruplex (G4); dsDNA
• Online Access: https://hdl.handle.net/10356/149750
• Institution: Nanyang Technological University

We generated a novel G-quadruplex (G4)-specific endonuclease by fusing a G4 recognition domain of the RHAU helicase with a cleavage domain of the Fok1 nuclease. The fusion protein can specifically bind a parallel G4 and cleave a double-stranded DNA (dsDNA) next to it. The new endonuclease could be used to detect a G4 in a long dsDNA, providing a useful tool for mapping the formation of G4s in the genome.