Design and synthesis of novel nucleotides for enzymatic gene synthesis
Traditionally, DNA was synthesized using a four-step chemical method based on phosphoramidite chemistry, allowing the synthesis of DNA strands up to 250-300 base pairs. However, this is insufficient for future application, such as DNA storage and gene synthesis. Alternatives to the phosphoramidite...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/152672 |
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| Institution: | Nanyang Technological University |
| Language: | English |