Design and synthesis of novel nucleotides for enzymatic gene synthesis
Traditionally, DNA was synthesized using a four-step chemical method based on phosphoramidite chemistry, allowing the synthesis of DNA strands up to 250-300 base pairs. However, this is insufficient for future application, such as DNA storage and gene synthesis. Alternatives to the phosphoramidite...
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Main Author: | Ee, Pin Koon |
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Other Authors: | Chiba Shunsuke |
Format: | Thesis-Doctor of Philosophy |
Language: | English |
Published: |
Nanyang Technological University
2021
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Subjects: | |
Online Access: | https://hdl.handle.net/10356/152672 |
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Institution: | Nanyang Technological University |
Language: | English |
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